Fig. 6: FK228 activates endoplasmic reticulum (ER) stress of solid tumor cells resulting in necroptosis.
From: FK228 reshapes tumor microenvironment to enhance anti-PD-L1 efficacy
A Standardized mean levels of endoplasmic reticulum stress related signatures in tumor cells grouped by FK228 pre- and post-treatment. Ctrl indicates DMSO treatment. B Western blotting showing the classic hallmarks expression of ER stress. C The cell viability of solid tumors treated with FK228 and subsequently recovered with 4-Phenylbutyric acid (4-PBA) using the CCK-8 assay. Solid tumor cells were exposed to FK228 at its IC50 concentrations and/or 4-PBA at doses of 10 μM or 50 μM for a duration of 24 h. Following treatment, cell survival was evaluated using the CCK-8 assay. Significant difference was executed using Student’s t-test. D Solid tumor cell death by FK228-induction after 4-PBA rescue using 7-AAD staining. Significant difference was executed using Student’s t-test. E Western blotting showing ER stress-mediated necroptosis.
