Fig. 1: Large-scale screening identified the novel micropeptide XLH-36 in triple-negative breast cancer. | Oncogene

Fig. 1: Large-scale screening identified the novel micropeptide XLH-36 in triple-negative breast cancer.

From: The novel endogenous micropeptide XLH-36 binds Gemin4 to promote triple-negative breast cancer metastasis

Fig. 1

A Schematic screening of differential expressed of potential coding lncRNAs from TCGA and the bioinformatic database. B Venn diagrams showing differentially expressed lncRNAs overlaps between breast cancer, TNBC samples, and coding ability analysis. C C5orf66-AS1, encoding XLH-36, was significantly upregulated in BRCA patients (n = 1090) compared to adjacent normal tissues (n = 113) from TCGA. D The GSE76250 dataset indicated that C5orf66-AS1 was significantly upregulated in TNBC patients (n = 165) compared to adjacent normal tissues (n = 33). E XLH-36-encoding C5orf66-AS1 expression in breast cancer cells and normal cells. Expression levels were normalized to GAPDH. F Diagram of the location XLH-36-encoding region in C5orf66-AS1. The XLH-36-Flag fusion protein was expressed in MDA-MB-231 cells and detected by immunoblot (G) and immunofluorescence analysis (H). Nuclei were stained with DAPI (blue). I XLH-36 expression was detected in breast cancer cells and normal cells were detected by immunoblot with prepared anti-XLH-36 antibody. J Amino acid alignment of XLH-36 from different vertebrates. Data are presented as mean ± SD from n = 3 biologically independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001.

Back to article page