Fig. 3: XLH-36 functions as an oncomicropeptide to promote TNBC metastasis in vitro and in vivo. | Oncogene

Fig. 3: XLH-36 functions as an oncomicropeptide to promote TNBC metastasis in vitro and in vivo.

From: The novel endogenous micropeptide XLH-36 binds Gemin4 to promote triple-negative breast cancer metastasis

Fig. 3

A Upper: Schematic diagram of the CRISPR/Cas9 based strategy to produce XLH-36-KO MDA-MB-231 and BT549 cells. Lower: Immunoblot of XLH-36 in wild-type and XLH-36-KO MDA-MB-231 and BT549 cells. B Cell proliferation was evaluated by the CCK-8 assay in the indicated cells. C Flow cytometry analysis of the cell cycle distribution in the indicated cells. D qPCR analysis of the cell cycle marker in the WT and XLH-36-KO MDA-MB-231 and BT549 cells. Cell metastatic properties were assessed by the Transwell migration assay (E) invasion assay (F) and wound scratch assay (G). H Representative images and statistical analysis of lung metastases of tumor-bearing mice after i.v. injection of the indicated MDA-MB-231 cells. In vivo-generated tumors and analyses of tumor volume (I) are shown. Data are presented as mean ± SD from n = 5 mice per group. Two-way ANOVA analysis. Lines show the means ± SD. *P < 0.05, **P < 0.01, ***P < 0.001.

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