Fig. 3: Bladder-specific Sqle manipulation modulates tumorigenesis in mouse models.
From: SQLE drives bladder cancer progression by boosting mitochondrial oxidative phosphorylation
Subcutaneous tumors derived from J82 cells overexpressing SQLE analyzed for tumor volume (A, B) and weight (C). D Western blots analysis of PCNA and cyclin D1 expression in subcutaneous tumors derived from J82 cells with or without SQLE overexpression. Subcutaneous tumors derived from UMUC3 cells with SQLE knockout analyzed for tumor volume (E, F) and weight (G). H Western blots analysis of PCNA and cyclin D1 expression in subcutaneous tumors derived from UMUC3 cells with or without SQLE knockout. I A schematic diagram depicting the experimental design for the induction of bladder cancer in Rosa26-Sqle mice and Sqle bladTg mice utilizing 0.05% BBN is presented. J Representative MRI imaging, gross histology, and H&E staining (left), along with tumor incidence and severity (right) of the bladder in Sqle bladTg mice (n = 5) compared to Rosa26-Sqle mice (n = 6) mice. K Representative Ki-67 staining in the bladders of BBN-treated Rosa26-Sqle mice (n = 6) versus Sqle bladTg mice (n = 5). L Western blots analysis of PCNA and cyclin D1 expression in bladder tissues from BBN-treated Rosa26-Sqle mice versus Sqle bladTg mice. M A schematic diagram illustrating the experimental design for the induction of bladder cancer in Sqlefl/fl mice and Sqle bladKO mice using 0.05% BBN is shown. N Representative MRI imaging, gross histology, and H&E staining (left), along with tumor incidence and severity (right) of the bladder in Sqle bladKO mice (n = 6) compared to Sqlefl/fl mice (n = 6). O Representative Ki-67 staining in the bladders of BBN-treated Sqlefl/fl mice (n = 6) versus Sqle bladKO mice (n = 6). P Western blots analysis of PCNA and cyclin D1 expression in bladder tissues from BBN-treated Sqlefl/fl mice versus Sqle bladKO mice. Data are expressed as means ± SD. *P < 0.05, **P < 0.01, ***P < 0.001. Paired, two-tailed Student’s t-test (B, F). Unpaired, two-tailed Student’s t-test (C, G, K, O). Scale bars, 500 µm (low magnification) and 200 µm (high magnification) (J, N). Scale bars, 200 µm (K, O).
