Fig. 3: Aggressive growth of EL4 cells in FGF21KO-MASH liver.
From: Aberrant lipid metabolism renders an aggressive behavior of T-lymphoblastic lymphoma in a MASH model
A Schematic diagram of establishing a hepatic lymphoma model in WT mice and FGF21KO mice. The mice were fed with HFMCD or CD for 2 weeks, then orthotopic inoculation was performed by EL4 cell injection in the liver lobe. Arrowhead: tumor nodules. B The gross anatomy of tumor nodules, histology by H&E staining, and proliferation by PCNA IHC staining in tumor/liver tissues from four experimental groups (WT-CD, WT-HFMCD, FGF21KO-CD, and FGF21KO-HFMCD). C Numbers of tumor nodules were counted macroscopically in the liver lobs from four experimental groups. D Computer-imaging analysis in IHC slides to calculate the indexes of PCNA positive cells from four experimental groups. E Immunofluorescent staining using the antibodies of anti-CD3 and anti-CD133 to detect EL4 cell growth in the tumor tissue from a FGF21KO-MASH-lymphoma mouse. F Analysis of extranodal infiltration (Ex) in stomach, lung, spleen, and skin from four experimental groups. G, H Western blotting analysis to detect the protein levels of β-catenin and EpCAM in the liver tissues from four experimental groups. Animal number (n) = 6 in each group; 21KO: FGF21KO.
