Fig. 4: Wnt/β-catenin signaling promoted EL4 cell growth.
From: Aberrant lipid metabolism renders an aggressive behavior of T-lymphoblastic lymphoma in a MASH model
A, B Western blot analysis for protein levels of AXIN-1 and phosphorylated β-catenin at thr41/ser45 from the OP-challenged EL4 cells treated with 1 μM XAV939 treatment at different time points, up to 24 h. C, D Western blot analysis for protein levels of phosphorylated GSK-3β at ser9 and phosphorylated β-catenin at thr41/ser45 from the OP-challenged EL4 cells treated with LiCl at different concentrations for 16 h. E Western blot analysis for protein levels of cyclin D1 from the OP-challenged EL4 cells treated with 20 mM LiCl and 1 μM XAV939 for 16 h. F Colony forming assay to detect the number of OP-challenged EL4 cells treated with 20 mM LiCl and 1 μM XAV939 for 16 h. G Trans-well assay to determine the migratory ability of EL4 cells co-cultured with OP-challenged FL83B-21KD cells or with OP-challenged FL83B-shCT cells, which were treated with 20 mM LiCl and/or 1 μM XAV939 for 16 h. H–J Western blot analysis for protein levels of β-catenin, EpCAM, and cyclin D1 from the EL4 cells co-cultured OP-challenged FL83B-21KD cells or with OP-challenged FL83B-shCT cells, which were treated with rhFGF21 at 100 ng/mL. OP: a mixture of oleic acid and palmitic acid (2:1); XAV: XAV939; p-β-Cat: phosphorylated β-catenin; p-GSK-3β: phosphorylated GSK-3β.
