Fig. 5: c-MYC promotes ECM-associated genes transcription and PI3K-AKT-mTOR activation in NSCLC. | Oncogene

Fig. 5: c-MYC promotes ECM-associated genes transcription and PI3K-AKT-mTOR activation in NSCLC.

From: POM121 O-GlcNAcylation facilitates bone metastasis in non-small cell lung cancer through enhanced c-MYC nuclear import and ECM reprogramming

Fig. 5

A Gene Ontology (GO) analysis of biological processes in H460-BMPOM121-KD cells and H460-BM cells. Top 5 significantly enriched pathways from RNA-seq data (p < 0.05). B Extracellular matrix (ECM)-related genes mRNA levels were analyzed by RT-qPCR between H460-BM and H460-BMPOM121-KD cells. (mean ± SEM; n = 3; unpaired t-test). C ECM-related genes mRNA levels were analyzed by RT-qPCR between H460-BM and H460-BMc-MYC-KD cells. (mean ± SEM; n = 3; unpaired t-test). D ECM-related genes mRNA levels were analyzed by RT-qPCR between H460-BM and H460-BMOGT-KD cells. (mean ± SEM; n = 3; unpaired t-test). E Immunoblot analysis of PI3K-AKT-mTOR pathway activation (p-PI3K/PI3K, p-AKT/AKT, p-mTOR/mTOR) between H460-BM and H460-BMPOM121-KD cells. Data representative of three experiments. F Immunoblot analysis of PI3K-AKT-mTOR pathway activation in H460-BM cells transfected with NC or si-c-MYC. Data representative of three experiments. G Immunoblot analysis of PI3K-AKT-mTOR pathway activation in H460-BM cells transfected with NC or OGT siRNAs. Data representative of three experiments. H Correlation between POM121 and ECM-related genes expression in NSCLC patients (TCGA database). (http://timer.comp-genomics.org). Spearman correlation test rho and p-values are shown.

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