Fig. 1: Characterization of lncRNA1456 and its encoded peptide, XCP.

A Genome browser view for the lncRNA1456 locus from fractionated RNA-seq data (cytoplasm vs nuclear) in the presence or absence of estrogen (E2) [19]. B Evolutionary Conserved Regions (ECR) browser tracks showing conservation of lncRNA1456 locus across different species. C Diagram showing the putative lncRNA1456-encoded peptide (XCP) from exons 1 and 2 and amino acid sequence. D Coomassie gel showing recombinant XCP peptide (left) and its endogenous detection in MCF-7 cells by Western blot using a derived XCP antibody (right). E Western blot showing reduced levels of endogenous XCP peptide upon siRNA-mediated knockdown of lncRNA1456 RNA in MCF-7 cells. β-tubulin was used a loading control. [See also Fig. S1].