Fig. 5: XCP interacts with the demethylase PHF8. | Oncogene

Fig. 5: XCP interacts with the demethylase PHF8.

From: X-linked cancer-associated polypeptide (XCP) from lncRNA1456 modulates PHF8 histone demethylase activity to regulate the epigenome, gene expression, and cellular pathways in breast cancer

Fig. 5: XCP interacts with the demethylase PHF8.

A Western blot showing expression of GFP and FLAG-tagged XCP used for pull down assay from MCF-7 whole cell lysates. B Western blot showing interaction of PHF8 and XCP confirmed in an in vitro pull down assay using recombinant FLAG-PHF8 and His6-XCP. C GTEx survey of PHF8 expression across normal human tissues. Observed differences are significant as determined by an ANOVA comparison of the means (p-value < 2.2 e-16). D Graph showing PHF8 RNA expression in normal solid tissue (TCGA), normal breast tissue (GTEx) and malignant primary breast tumors (TCGA). Observed differences are significant as determined by an ANOVA comparison of the means. E PHF8 RNA expression in malignant primary breast tumors (TCGA) stratified into subtypes using PAM50 gene set analysis compared to normal breast tissue (GTEx). Observed differences are significant as determined by an ANOVA comparison of the means. [See also Fig. S4].

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