Fig. 5: Effect of MKD on motility, invasiveness, and proliferation.

Effect of MKD on motility of HCE4 cells (a) or TE2-CK2α cells (b). Motility was measured with the OrisTM Cell Migration Assay kit. *P < 0.05, **P < 0.01 compared to the C. Effect of MKD on invasion of HCE4 cells (c) or TE2-CK2α cells (d). The cells were incubated for 48 h in the upper chambers coated with Matrigel. Data represent the mean ± SD of one experiment performed in triplicate. Similar results were obtained from two independent experiments. *P < 0.05 compared to the C. Effect of MKD on proliferation of HCE4 cells (e) or TE2-CK2α cells (f). Cells were counted at 24 and 48 h after seeding. Data represent the mean ± SD of one experiment performed in triplicate. Similar results were obtained from two independent experiments. C represents scrambled shRNA and M represents MZF1 shRNA, respectively