Fig. 5: MIR222HG expression pattern in normal prostate, primary tumors, and CRPC.
a Comparison of the MIR222HG expression. Expression levels of MIR222HG were measured by quantitative RT-PCR on 86 normal prostate tissues (black dots), 34 hormone-sensitive primary prostate tumors (blue squares), and 17 metastatic CRPC tissues (red triangles). All expression levels were normalized by 28S ribosome RNA (RPS28). Values represent in qPCR cycle differences relative to the mean expression level of normal tissue, which was set as 0. P values were estimated by one-way ANOVA. b The expression correlation between MIR222HG and miR-221 or miR-222 in human prostate tissue specimen. The Pearson's correlation coefficient indexes (r2) were calculated by comparing normalized qPCR cycle values (∆cts) in detecting miR-221 (blue) or miR-222 (red) with ∆ct value of MIR222HG. c Association of expression levels of MIR222HG, miR-221, and miR-222 with GS in primary tumors of the TCGA data set (p < 0.0001 Kruskal–Wallis test). d Correlation of MIR222HG expression level with AR activity (indicated by AR score) in primary tumors of the TCGA data set. AR activity score was inferred by the induction of AR target genes24,25. AR score Nelson indicates that the AR score was re-evaluated using the AR gene index previously published by Dr. Nelson’s laboratory25. e Correlation of GS with AR activity score
