Fig. 2: Notch3 downregulation by siRNA affects TNBC cells survival. | Oncogenesis

Fig. 2: Notch3 downregulation by siRNA affects TNBC cells survival.

From: NOTCH3 inactivation increases triple negative breast cancer sensitivity to gefitinib by promoting EGFR tyrosine dephosphorylation and its intracellular arrest

Fig. 2

a, d Analysis of cell growth after 0–3–6 days of Notch3 and Notch1 silencing in a MDA-MB-468 and d BT-549 cells. b, c Whole cell extracts from a MDA-MB-468 or d BT-549 cells at 6 day of silencing were used for western blot against Notch3 (N3IC) and Notch1 (N1IC), to control the efficiency of the b, e Notch3 and c, f Notch1 silencing, respectively. Extracts were then immunoblotted with anti-p27, anti-cyclin D1, and anti-cyclin D3 antibodies. Anti-β-actin was used as a loading control. b, c, e, f are representative of three separate experiments. The statistical analysis associated is available in the Supplementary Figure S2

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