Fig. 2: Downregulation of UBE2C reduces cell proliferation, clonogenicity, and invasive growth of NSCLC.
From: Deregulation of UBE2C-mediated autophagy repression aggravates NSCLC progression
a siRNA-induced specific downregulation of UBE2C at both mRNA and protein levels in A549 cells as shown in RT-PCR and immunoblotting. b Knockdown of UBE2C reduced clonal formation of A549 cells in the representative images of colony formation with quantitative clonogenicity assay. c Quantitative scratch assay showing that knockdown of UBE2C dramatically slowed cell migration in A549 cells. d SA-β-Gal assay showing that transient knockdown of UBE2C for 36 h significantly increased cell senescence phenotype in A549 cells. e Matrigel invasion assay indicated downregulation of UBE2C significantly decreased cell invasive growth and migration of A549 cells. f–h Immunofluorescent staining and immunoblotting of UBE2C and eminent EMT markers demonstrating that knockdown of UBE2C (f) enhanced E-cadherin (g, h) expression while repressing vimentin (g, h) in A549 cells. **P < 0.001, ***P < 0.0001 by Student’s t test
