Fig. 6: PDGFR-α/β signaling in sarcoma CSCs is upregulated by hypoxia. | Oncogenesis

Fig. 6: PDGFR-α/β signaling in sarcoma CSCs is upregulated by hypoxia.

From: RETRACTED ARTICLE: Platelet-derived growth factor receptor-α and -β promote cancer stem cell phenotypes in sarcomas

Fig. 6

a Western blot analysis of HIF-1α, phosphorylated and total PDGFR-α and -β, and CD133 in human sarcoma cell lines grown as monolayers or as spheroids under normoxia (21% O2) and hypoxia (1% O2) for 18–24 h. b Graph displaying the mean numbers of spheroids >50 μm per field formed by sarcoma spheroid cells under normoxia and hypoxia. c Graphs displaying the mean numbers of spheroids >50 μm per field formed by sarcoma spheroid cells under normoxia and hypoxia following transduction with control shRNA (sh.Scr) or PDGFR-α and -β shRNA (sh.PDGFR-α/β). d Western blot analysis of HIF-1α, phosphorylated and total PDGFR-α and -β in human sarcoma spheroid cells under normoxia (21% O2) and hypoxia (1% O2) following transduction with control shRNA (sh.Scr) or HIF-1α shRNA (sh.HIF-1α). Experiments a and d were performed three times with similar results. Bars represent standard deviation. *p < 0.05 compared to Normoxia

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