Fig. 5: Aneuploidy-induced senescence promotes SASP.
From: Chromosomal instability-induced senescence potentiates cell non-autonomous tumourigenic effects
a qRT-PCR of SASP components and pro-tumourigenic factors in RPE-1 cells 5 days (D5) after mitotic shake-off from 8 h or 16 h Noc treatment. mRNA levels were normalised to actin. b, c qRT-PCR of SASP factors in (b) RPE-1 cells or (c) HCT116 cells 5 days (D5) following Rev washout. d Experimental schema of conditioned media (CM) preparation for Luminex cytokine assay. e Cytokine levels in CM of control and 16 h Noc-treated RPE-1 cells determined by Luminex cytokine assay. All data are expressed as mean ± SD from three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. NS not significant by Student’s t-test
