Fig. 1: MEK inhibitor induces apoptosis in CRC cells. | Oncogenesis

Fig. 1: MEK inhibitor induces apoptosis in CRC cells.

From: RETRACTED ARTICLE: MEK inhibitors induce apoptosis via FoxO3a-dependent PUMA induction in colorectal cancer cells

Fig. 1

a The indicated cell lines were treated with increasing doses of trametinib or selumetinib for 72 h. Cell proliferation was determined by MTS assay. b The indicated cell lines were treated with 0.1 μmol/L trametinib or 0.1 μmol/L selumetinib for 24 h at the indicated concentrations. Apoptosis was analyzed by Annexin V/PI staining followed by flow cytometry. c The indicated cell lines were treated with 0.1 μmol/L trametinib or 0.1 μmol/L selumetinib for 24 h at the indicated concentrations. Caspase 3/7 activity was determined by fluorogenic analysis. d The indicated cell lines were treated with 0.1 μmol/L trametinib or 0.1 μmol/L selumetinib at the indicated time points. Cleaved caspase 3, 8, and 9 were analyzed by Western blotting. The results in a, b, and c are expressed as the means ± SD of three independent experiments. **P < 0.01, *P < 0.05

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