Fig. 2: MEK inhibitors induce p53-independent PUMA induction. | Oncogenesis

Fig. 2: MEK inhibitors induce p53-independent PUMA induction.

From: RETRACTED ARTICLE: MEK inhibitors induce apoptosis via FoxO3a-dependent PUMA induction in colorectal cancer cells

Fig. 2

a Parental and p53-KD RKO cells were treated with 0.1 μmol/L trametinib or 0.1 μmol/L selumetinib for 24 h. PUMA mRNA induction by trametinib was analyzed by real-time reverse transcriptase (RT) PCR, with β-actin as the control. b RKO cells were treated with 0.1 μmol/L trametinib at the indicated time points. PUMA expression was analyzed by Western blotting. c RKO cells were treated with 0.1 μmol/L selumetinib at the indicated time points. PUMA expression was analyzed by Western blotting. d Parental and p53-KD RKO cells were treated with 1 μmol/L trametinib at the indicated time points. PUMA expression was analyzed by Western blotting

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