Fig. 4: Induction of PUMA by MEK inhibitors is mediated through ERK inhibition. | Oncogenesis

Fig. 4: Induction of PUMA by MEK inhibitors is mediated through ERK inhibition.

From: RETRACTED ARTICLE: MEK inhibitors induce apoptosis via FoxO3a-dependent PUMA induction in colorectal cancer cells

Fig. 4

a RKO cells were treated with 0.1 μmol/L trametinib for 24 h. PUMA, p73, E2F1, p-FoxO3a and FoxO3a expression were analyzed by Western blotting. b RKO cells were treated with 0.1 μmol/L trametinib or 0.1 μmol/L selumetinib for 24 h. Indicated protein levels were analyzed by Western blotting. c RKO cells were transfected with ERK plasmid for 6 h and then treated with 1 μmol/L trametinib for 24 h. PUMA and ERK expression was analyzed by Western blotting. d RKO cells were transfected with either a control scrambled siRNA or FoxO3a siRNA for 24 h and then treated with 1 μmol/L trametinib for 24 h. FoxO3a and PUMA expression was analyzed by Western blotting. e Chromatin immunoprecipitation (ChIP) was performed using an anti-FoxO3a antibody on RKO cells following trametinib treatment for 12 h. ChIP with the control IgG was used as a control. PCR was carried out using primers surrounding the FoxO3a binding sites in the PUMA promoter

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