Fig. 6: PUMA mediates the anticancer effects of trametinib in a xenograft model. | Oncogenesis

Fig. 6: PUMA mediates the anticancer effects of trametinib in a xenograft model.

From: RETRACTED ARTICLE: MEK inhibitors induce apoptosis via FoxO3a-dependent PUMA induction in colorectal cancer cells

Fig. 6

a Nude mice were injected s.c. with 4 × 106 parental and PUMA-KD RKO cells. After 1 week, mice were treated with 1 mg/kg trametinib or buffer for ten consecutive days. The tumor volume at the indicated time points after treatment was calculated and plotted (n = 6 in each group). Arrows indicate trametinib injection. The results are expressed as the means ± SD of three independent experiments. **P < 0.01; *P < 0.05. b Parental RKO xenograft tumors were treated with 1 mg/kg trametinib or the control buffer as in a for four consecutive days. The indicated protein in representative tumors was analyzed by Western blotting. c Paraffin-embedded sections of tumor tissues from mice treated as in b were analyzed by TUNEL staining. d Tissue sections from c were analyzed by active caspase 3 staining. Scale bars 25 μm

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