Fig. 3: Snail regulates fibroblast alignment, matrix remodeling and tubulogenesis in response to PDGF. | Oncogenesis

Fig. 3: Snail regulates fibroblast alignment, matrix remodeling and tubulogenesis in response to PDGF.

From: Endothelial cell activation on 3D-matrices derived from PDGF-BB-stimulated fibroblasts is mediated by Snail1

Fig. 3: Snail regulates fibroblast alignment, matrix remodeling and tubulogenesis in response to PDGF.

a SNAI 1 expression is required for alignment of fibroblasts dependent on PDGF ligand. Below the representative images the directionality histograms, calculated with the Image J software, show the frequency of distribution of cell angles (centered on the 0 ° angle). b, c Increase in ECM fiber deposition (b) and Organization (c) in matrices derived from PDGF-stimulated fibroblasts were dependent on SNAI1 expression. Under the image its represented the directionality histograms calculated with the Image J software, representing the frequency of distribution of protein fibers angles (centered on the 0° angle). d, e Formation of capillary-like structures on matrices derived from PDGF-stimulated fibroblasts (d) and increased expression of angiogenesis-related markers (e) are blocked in SNAI1 KO fibroblasts. f Down-regulation of β3 integrins in HUVECs from SNAI1 KO fibroblasts matrices. All results are derived from 2–4 independent experiments, each performed in duplicate

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