Fig. 3 | Oncogenesis

Fig. 3

From: Activation of c-Met in cancer cells mediates growth-promoting signals against oxidative stress through Nrf2-HO-1

Fig. 3

Silencing of Nrf2 prevents the protective role of c-Met against sorafenib-induced DNA damage and the downregulation of anti-oxidant HO-1. a, b 786-O cells were transfected with Nrf2 siRNA (50 nM)/control siRNA for 48–72 h. The transfected cells were subjected to combination treatments with HGF (50 ng/ml), sorafenib (10 μM), or only vehicle for 24 h. The cell lysates were utilized for checking the levels of the DNA damage marker γ-H2AX (a) and the redox protective enzyme HO-1 (b) β-Actin (internal control) by Western blot. β-Actin was measured as internal control. a, b Results are representative of three different experiments

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