Fig. 4: GSK-3b is a downstream target of ILK upon FGFR inhibition. | Oncogenesis

Fig. 4: GSK-3b is a downstream target of ILK upon FGFR inhibition.

From: A functional CRISPR/Cas9 screen identifies kinases that modulate FGFR inhibitor response in gastric cancer

Fig. 4: GSK-3b is a downstream target of ILK upon FGFR inhibition.

a KatoIII cells treated with vehicle control, AZD4547, Cpd22, or AZD4547/Cpd22 for 1 h. Four hundred micrograms of cell lysates were incubated with membrane to detect phosphorylation of 43 kinases and two total proteins using human phospho-kinase array (R&D Systems) according to manufacturer’s instruction. Each kinase was detected in duplicate. Reference spots labeled with the white box. b Western blot of the cell lysates used in (a), probed for p-GSK-3b (Ser9), total GSK-3b, p-AKT (Ser473), and total AKT. Vinculin is used as the loading control

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