Fig. 6: Fibroblast growth factor receptor 1 (FGFR1) regulates the expression of miR-214 by regulating extracellular signal-regulated kinase (ERK).
From: Reciprocal regulatory mechanism between miR-214-3p and FGFR1 in FGFR1-amplified lung cancer
a After treatment with FGF2 or FGFR1 inhibitor AZD4547 (1 μM) or FGF2 and AZD4547 in H1581 and DMS114 cell lines, the level of miR-214-3p was detected by quantitative real-time PCR (qRT-PCR). b After treatment with MEK/ERK inhibitor AZD6244 (1 μM) or FGF2 or FGF2 and AZD6244 (1 μM) in H1581 and DMS114 cell lines, the level of miR-214-3p was detected by qRT-PCR. c, d H1581 and DMS114 cell lines were transfected by plasmid-NC, wild-type ERK2 plasmid (plasmid-ERK2), or autophosphorylation variant ERK2_R67S plasmid (plasmid-ERK_R67S), or plasmid-ERK_R67S followed by treatment of AZD4547 or AZD6244. c Expression of pERK1/2 and total ERK1/2 were detected by Western blot. d Expression of miR-214-3p were measured by qPCR. P values were calculated by Student’s t test: **p < 0.01; ***p < 0.001; ****p < 0.0001
