Fig. 4: Effects of CK2 inhibition on HUCCT-1 and CCLP-1 motility and invasiveness.
From: The protein kinase CK2 contributes to the malignant phenotype of cholangiocarcinoma cells
Cell migration (a, b) or invasiveness (c, d) were evaluated using Boyden chamber assay with 10% FBS as chemotactic stimulus, in the presence or absence of 5 μM CX4945. Before the assay, CCA cells were serum-starved for 24 h. *p < 0.05 vs untreated cells, **p < 0.05 vs. FBS-stimulated cells. e Wound-healing assay. HUCCT-1 cells were plated at confluency, scratched with a pipette tip and allowed to heal the wound in 10% FBS-containing medium, in the presence or absence of CX4945 5 µM for 6 h. Images were taken at the end of the experiment (original magnification ×200) and the wound area was quantified using Adobe Photoshop® software. Scale bar corresponds to 200 µm. Data are expressed as the empty area (mean ± SEM) from three independent experiments. *p < 0.05 vs FBS
