Fig. 6: Effects of CK2α knockout on CCA cell biology.
From: The protein kinase CK2 contributes to the malignant phenotype of cholangiocarcinoma cells
a Cellular lysates of wild type (WT) and a CK2α knockout clone (KO CK2α) were analyzed by western blot with the indicated antibodies. b WT and KO CK2α cells were serum-starved for 24 h and incubated in the presence of 10% FBS for 24 h. Proliferation rate was measured by bromodeoxyuridine (BrdU) incorporation assay. *p < 0.05 vs WT cells treated with FBS. Cell migration (c) or invasiveness (d) in WT and KO CK2α cells were evaluated using a Boyden chamber assay, with FBS as chemotactic stimulus. Cells were serum-starved for 24 h before the assay. *p < 0.05 vs control cells, **p < 0.05 vs FBS-stimulated cells. e, f Viability of WT and KO CK2α cells was assessed by MTT after treatment with increasing concentrations of 5-fluorouracil for 48 h or gemcitabine for 72 h. *p < 0.05 vs WT cells treated with the indicated drug at the same concentration
