Fig. 3: Expression profiling of melanoma cells with induction of BRN2 expression.
From: BRN2 expression increases anoikis resistance in melanoma
a, b Entities at least 1.5 fold a up- or b downregulated following induction of BRN2 were identified by firstly comparing to the same uninduced cells, then by comparing to lacZ-expressing cells versus uninduced cells to account for changes potentially caused by protein expression or doxycycline exposure. Full data are in Supplementary Tables S1 and S2. c qPCR validation of targets following induction of BRN2 or lacZ expression after 48 h exposure to doxycycline. Values indicate mean ± SEM; n = at least two independent experiments with at least triplicate reading. d Western blot analysis of targets following induction of BRN2 or lacZ expression after 48 h exposure to 50 ng/ml doxycycline or vehicle. Representative blots of two independent experiments are shown.
