Fig. 3: MRTF-A directly regulates TCL transcription. | Oncogenesis

Fig. 3: MRTF-A directly regulates TCL transcription.

From: Epigenetic activation of the small GTPase TCL contributes to colorectal cancer cell migration and invasion

Fig. 3

a HEK293 cells were transfected with a TCL promoter-luciferase construct and increasing doses of MRTF-A. Luciferase activities were normalized by protein concentration and GFP fluorescence and expressed as relative luciferase unit compared to the control group. b HEK293 cells were transfected with a TCL promoter-luciferase construct and increasing doses of dominant-negative (DN) MRTF-A. Luciferase activities were normalized by protein concentration and GFP fluorescence and expressed as relative luciferase unit compared to the control group. c HEK293 cells were transfected with a TCL promoter-luciferase construct and increasing doses of CCG-1423. Luciferase activities were normalized by protein concentration and GFP fluorescence and expressed as relative luciferase unit compared to the control group. d HT-29 cells were exposed to 1% O2 and harvested at indicated time points. ChIP assays were performed with anti-MRTF-A or IgG. Data represent averages of three independent experiments and error bars represent SEM.

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