Fig. 4: MRTF-A modulates histone H4K16 acetylation surrounding the TCL promoter. | Oncogenesis

Fig. 4: MRTF-A modulates histone H4K16 acetylation surrounding the TCL promoter.

From: Epigenetic activation of the small GTPase TCL contributes to colorectal cancer cell migration and invasion

Fig. 4

a–f HT-29 cells were exposed to 1% O2 and harvested at indicated time points. ChIP assays were performed with anti-acetyl H3 (a), anti-acetyl H4 (b), anti-acetyl H4K5 (c), anti-acetyl H4K8 (d), anti-acetyl H4K12 (e), and anti-acetyl H4K16 (f). g HT-29 cells were transfected with siRNA targeting MRTF-A or scrambled siRNA (SCR) followed by treatment with 1% O2 for 48 h. ChIP assays were performed with anti-acetyl H4K16. h HT-29 cells were treated with 1% O2 in the presence or absence of CCG-1423 for 48 h. ChIP assays were performed with anti-acetyl H4K16. Data represent averages of three independent experiments and error bars represent SEM.

Back to article page