Fig. 2: MiR-22 inhibits AGS and MKN45 cell growth and induced apoptosis by targeting MeCP2, MTHFD2, and MTHFR. | Oncogenesis

Fig. 2: MiR-22 inhibits AGS and MKN45 cell growth and induced apoptosis by targeting MeCP2, MTHFD2, and MTHFR.

From: MiR-22, regulated by MeCP2, suppresses gastric cancer cell proliferation by inducing a deficiency in endogenous S-adenosylmethionine

Fig. 2: MiR-22 inhibits AGS and MKN45 cell growth and induced apoptosis by targeting MeCP2, MTHFD2, and MTHFR.The alternative text for this image may have been generated using AI.

a Relative expression of miR-22 in 39 pairs of clinical samples. b MTT assay of cell proliferation at 24, 48, and 72 h after transfection with miR-22 overexpression vector. c Cell proliferation was detected after transfection with miR-22 inhibitor. d Colony formation assay 14 days after transfection. e Flow cytometry analysis of cell cycle in AGS and MKN45 cells f Flow cytometry analysis of cell apoptosis after transfection with miR-22 overexpression vector or miR-22 inhibitor. g HEK-293T cells were co-transfected with the miR-22 overexpression vector and indicated reporter vector (control/WT/MUT), and the effects of miR-22 targeting MeCP2, MTHFD2, and MTHFR were determined by dual fluorescence report assay. h Expression of miR-22 in miR-22 overexpression vector-transfected AGS and MKN45 cells. i Expression of MeCP2, MTHFD2, and MTHFR in AGS and MKN45 cells transfected with miR-22 overexpression vector. j MeCP2, MTHFD2, and MTHFR protein levels were detected after miR-22 overexpression or inhibition, MeCP2 overexpression or knockdown. k Endogenous and exogenous MeCP2 level in GFP-tagged MeCP2 expression vector (MeCP2-GFP) transfected cells. The GFP has a molecular weight of ~27 KD, and the GFP/MeCP2 fusion protein is larger than endogenous MeCP2. All the results are shown as the mean ± SD. n = 3, *p < 0.05, **p < 0.01, Student’s t test.

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