Fig. 4: PBK promotes the formation of MSL complex on the promoter of CD276.

A Immunoprecipitation assays with MSL1 antibody and immunoblotting analyses with the indicated antibodies were performed in PBK-overexpression stable cell lines. Left, CNE1, right, HK1. B CNE2 and SUNE1 cells were transfected with PBK siRNAs or NC (nonspecific siRNA) for 48 h. Immunoprecipitation assays with MSL1 antibody and immunoblotting analyses with the indicated antibodies were performed. Left, CNE2, right, SUNE1. C ChIP-qPCR (chromatin immunoprecipitation-quantitative PCR) analysis of CD276 promoter using antibodies against MSL1 and KAT8 in PBK-overexpression or control cell lines. Left, CNE1, right, HK1. D ChIP-qPCR analysis of CD276 promoter using antibodies against MSL1 and MOF in PBK-knockdown or control cells. Left, CNE2, right, SUNE1. E, F ChIP-qPCR analysis of CD276 promoter using antibodies against H4K16ac (acetylation of histone H4 on lysine 16) in PBK-overexpression (E) or -knockdown cells (F). In C–F, ChIP-qPCR results were presented as the fold change over the vector/IgG or NC/IgG group. Error bars represent the mean ± SD (standard deviation) for three biological replicates. ***P < 0.001 (student’s t test for two groups, one-way ANOVA for three groups). All the experiments were performed independently three times with similar results and the data are representative of three independent experiments.