Fig. 1: DNMT3A-MT deregulates cell proliferation and clonogenicity in EOL-1 cells. | Oncogenesis

Fig. 1: DNMT3A-MT deregulates cell proliferation and clonogenicity in EOL-1 cells.

From: DNMT3A mutants provide proliferating advantage with augmentation of self-renewal activity in the pathogenesis of AML in KMT2A-PTD-positive leukemic cells

Fig. 1

a Immunoblot of DNMT3A overexpressed with WT and mutant-DNMT3A in KMT2A-PTD positive EOL-1 cells. b Growth curves of EOL-1 cells stably transduced with WT- and mutant-DNMT3A. Representative results from three independent replicates are shown. c, d Colony-formation ability in methylcellulose containing medium after stable overexpression of WT; and mutant-DNMT3A in EOL-1 cells (original magnification: ×100). Colonies of more than 50 cells were scored on day 10 of cultures. e Cell viability of transformed EOL-1 cells in the presence of 200 nM ATRA, 600 nM SAHA and the combination of 100 nM ATRA with 500 nM SAHA at 72 h. Error bars represent ± s.d. of the mean of duplicate cultures and each experiment repeated at least three times. *P < 0.05, **P < 0.03, ***P < 0.01, either compared with the control or as indicated in figures. Two-sided Student’s t test was used to calculate the P value.

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