Fig. 1: FIRΔexon2, but not FIR, reduced the level of H3K27ac of BRG1.
From: Post-transcriptional regulation of BRG1 by FIRΔexon2 in gastric cancer
a Genome structure of PUF60, FIR, and FIRΔexon2 are indicated. The FIR/PUF60 gene is located at 8q24.3 and contains 12 exons. Primers and probes used for RT-PCR are indicated. PUF60 consists of 559 amino acids (a.a.) and FIR, lacking exon5, is 542 a.a. FIRΔexon2 lacking exon2 of transcriptional repression domain, is 513 a.a. RNA recognition motif and U2AF-homology motif (UHM) are indicated. The 6B4 (Supplementary Table S5) monoclonal antibody recognizes the amino-terminus of FIR family (arrow). FIR family, consisting of FIR, PUF60 and FIRΔexon2, could not be identified separately by the monoclonal antibody (6B4) used in this study. The list of co-immunoprecipitated proteins with FIR and FIRΔexon2 detected by a direct nanoflow liquid chromatography-tandem mass spectrometry analysis in 293 T cells and GeLC-MS of Flag-conjugated bead pull down with LC-MS in HeLa cells (b), c The overexpression of FIRΔexon2, dominant-negative of FIR, reduced the level of H3K27ac by 37% as compared to that in untreated sample in the BRG1 genome region, but its mRNA level remained unchanged. One possibility is that translational processes were interfered by FIRΔexon2 and its counterparts. d The protein expression of BRG1 was decreased by FIRΔexon2 overexpression.
