Fig. 3: Tumorigenesis and histological type of Gan-mouse and FIR+/− mouse.
From: Post-transcriptional regulation of BRG1 by FIRΔexon2 in gastric cancer
a, top panels Picture of Gan-mouse gastric tumor and FIR+/− mouse gastric tumor. a, middle panels hematoxylin-eosin staining photo (40 × ) of Gan-mouse and FIR+/− mouse gastric tumor. Note, lamina muscularis mucosae was intact (a, top and middle panels). a, bottom panels Wild-type mice showed no gastric tumors during the period of this experiment. Normal gastric mucosa was used as a negative control. b Expression of FIR and related proteins was examined by western blotting in wild-type mice tissues, as well as in Gan-mouse and FIR+/− mice gastric tumor tissues. The expressions of FIR family, Snai1, BRG1, E-cadherin, FBW7, c-Myc, cyclin-E, SAP155, and hnRNPA1 of wild-type, Gan-mice, and FIR+/− mice were examined. Frozen tissues samples of mice gastric tumors were obtained and proteins expression profiles examined by western blotting in three different genotypes: wild-type mouse (lanes 1–3), Gan-mice (gastric tumor wet weight < 0.5 g) (lanes 4–6), Gan-mice (gastric tumor wet weight > 2.0 g) (lanes 7–9) and FIR+/− mice (lanes 10–12). c The extent of the signals detected by western blotting revealed in Fig. 1 was quantified by densitometry. d Alternative splicing forms of PUF60 and FIR mRNAs were detected in NIH3T3 cells by reverse-transcription-polymerase chain reaction (RT-PCR).
