Fig. 6: TRPM2-AS promotes GC cell radioresistance by indirectly regulating FOXM1.

a The expression of TRPM2-AS was upregualted in GC cells received 2 Gy and 8 Gy dose of irradiation compared with paired untreated cells. b Radiation survive curves are shown for SGC7901 cells (left) and MGC803 cells (right) which were transduced with TRPM2-AS shRNA or scrambled control with a single increasing dose of 0, 2, 4, 6, 8, 10 Gy. Using ANOVA. c Radiation survive curves for SGC7901 cells (left) and MGC803 cells (right) which were transfected with miR-612 mimics or control. Using ANOVA. d Western blot analysis of γH2Ax expression in GC cells treated with TRPM2-AS shRNA or scrambled control (right), and treated with miR-612 mimics or control (left) after exposed to 8 Gy dose of radiation. e Radiation survive curves for SGC7901 and MGC803 cells cotransfected with TRPM2-AS shRNA, miR-612 inhibitor or negative control as indicated. Using ANOVA. f Radiosensitivity parameters of respective cell groups. D0, dose to reduce survival to 37%; SF2, surviving fraction at 2 Gy; SER10, sensitizer enhancement ratio at 10% survival. g GC cells were treated as indicated, then Survival fractions were calculated for each group after treatment with increasing dose of radiation. Using ANOVA. h Radiosensitivity parameters of respective cell groups. Error bars, mean ± SD. *P < 0.05; **P < 0.01.