Fig. 5: p53 rescues PIK3CD-AS2-induced LUAD cells proliferation and apoptotic resistance. | Oncogenesis

Fig. 5: p53 rescues PIK3CD-AS2-induced LUAD cells proliferation and apoptotic resistance.

From: The long non-coding RNA PIK3CD-AS2 promotes lung adenocarcinoma progression via YBX1-mediated suppression of p53 pathway

Fig. 5

a–c A549 cells were transfected with PIK3CD-AS2 siRNA, control siRNA, and p53 siRNA as the rescued group. After 24 h of transfection, cells were seeded as explained in Fig. 2c and monitored by RTCA for 90 h (a). ***P < 0.001 determined by unpaired t-test. After 48 h of transfection, cells were harvested and measured for apoptosis (b) and cell cycle phase distribution (c) using flow cytometry. d–f A549 cells were transfected with PIK3CD-AS2 expression plasmid, control plasmid, and p53 plasmid as the rescued group. Cells proliferation were monitored by RTCA (d). ***P < 0.001 determined by unpaired t-test. The apoptosis (e) and cell cycle phase distribution (f) were assessed by flow cytometry. Values represent mean ± SD of three independent experiments. Statistical analysis was carried out using unpaired t-test. *P < 0.05; ***P < 0.001.

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