Fig. 2: JQ1 treatment suppresses GC cell migration and invasion via inducing MET both in vitro and in vivo.

a The migratory capability of HGC27 and AGS cells treated with JQ1 (0, 200 nM, 500 nM, 1 μM, 2 μM, and 5 μM) for 72 h revealed by wound-healing assay. b The number of migrating and invading GC cells after treatment with JQ1 (0, 200 nM, 500 nM, 1 μM, 2 μM, and 5 μM) for 72 h by transwell assays. Representative images of migrated and invaded HGC27 and AGS cells in each group were shown on the left. Cells that migrated and invaded through the pores of transwell plates were counted in five random fields and were reported on the right (mean ± SEM, **p < 0.01, ***p < 0.001, ****p < 0.0001). c WB analysis of the expression of E-cadherin, Vimentin, and Snail in HGC27 and AGS cells after treatment with JQ1 (0, 200 nM, 500 nM, 1 μM, 2 μM, and 5 μM) for 72 h. d Immunofluoresence staining of the EMT markers Vimentin and E-cadherin in HGC27 and AGS cells after JQ1 (0, 1 μM, and 5 μM) treatment. e The macroscopic nodules in the peritoneal cavity in each group of mice (n = 6) were photographed. The number of the macroscopic nodules in each mouse was recorded (mean ± SEM, **p < 0.01). f The body weights of each mouse were recorded (mean ± SEM, p = 0.0117).