Fig. 5: JQ1 suppresses GC cell metastasis via mediating RUNX2/NID1 signaling in vitro.

a Luciferase reporter gene assay showed that the transcriptional activity of RUNX2 promoter in AGS cells was repressed by JQ1 (0, 200 nM, 500 nM, 1 μM, 2 μM, and 5 μM) (mean ± SEM, ****p < 0.0001). b ChIP-qRT-PCR analysis of BRD4 occupancy of the RUNX2 gene in AGS cells after JQ1 (0, 1 μM) treatment for 72 h (mean ± SEM, *p < 0.05). c, d RUNX2 overexpression significantly antagonized the downregulation of the migration and invasion induced by JQ1 in HGC27 and AGS cells. Representative images of the migrated and invaded GC cells in each group were shown on the left. Cells that migrated and invaded through the pores of transwell plates were counted in five random fields and were reported on the right (mmean ± SEM, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001). e RUNX2 overexpression significantly attenuated the downregulation of NID1 expression induced by JQ1 in HGC27 and AGS cells detected by WB analysis. f, g NID1 overexpression significantly repressed the downregulation of the migration and invasion induced by JQ1 in HGC27 and AGS cells. Representative images of the migrated and invaded GC cells in each group were shown on the left. Cells that migrated and invaded through the pores of transwell plates were counted in five random fields and were reported on the right (mean ± SEM, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001).