Fig. 4: Lack of Mme promotes Pten-deficient prostate stem/progenitor cell expansion and sphere-forming capacity. | Oncogenesis

Fig. 4: Lack of Mme promotes Pten-deficient prostate stem/progenitor cell expansion and sphere-forming capacity.

From: Membrane metalloendopeptidase suppresses prostate carcinogenesis by attenuating effects of gastrin-releasing peptide on stem/progenitor cells

Fig. 4

a Quantitative analysis of distribution of CD49fhi/Sca-1+ stem/progenitor cells and CD49flo/Sca-1− luminal cells isolated from three-month-old WT, Mme−/−, PtenPE−/−, and Mme−/−PtenPE−/− mice (n = 6 per genotype). Red and blue frames represent stem/progenitor cell and luminal cell populations, respectively. Frequency (b, d) and size (c) of spheres formed by CD49fhi/Sca-1+ stem/progenitor cells (b, c) and CD49flo/Sca-1− (d) luminal cells from 3-month-old WT, Mme−/−, PtenPE−/−, and Mme−/−PtenPE−/− mice (n = 6 per genotype). Relative frequency of sphere formation by CD49fhi/Sca-1+ stem/progenitor cells (e) and CD49flo/Sca-1− luminal cells (f) isolated from 3-month-old WT, Mme−/−, PtenloxP/loxP, and Mme−/−PtenloxP/loxP mice followed by Ad-Cre infection (n = 6 per genotype). Spheres counts were normalized to the Ad-blank-infected spheres of each passage. P0–P3, passages 0–3. **P < 0.01, ***P < 0.001. Error bars denote SD. a–f Data represent three independent experiments.

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