Fig. 4: LMW-E and CDK5 are co-expressed in human salivary gland tumors.

A Western blot analysis of cyclin E, in 18 salivary gland tumor samples from patients of different histological subtypes. Lane designations correspond to sample numbers given in Supplementary Table 1.231: MDA-MB-231 parental cells; MCF-T2: MCF-7 cells transfected with LMW-E (T2 isoform) B Analysis of cyclin E and CDK5 expression in different histologic subtypes of salivary duct carcinomas. Representative immunohistochemistry images for CDK5 and cyclin E are shown. Original magnification: ×200 (main images); ×800 (insets). Specifically, cyclin E (A1–D1) and corresponding CDK5 (A2–D2) staining for low cyclin E/CDK5; cyclin E (A3–D3) and corresponding CDK5 (A4–D4), for high CDK5/cyclin E expression. C Correlation analysis between high/low cyclin E (cytoplasmic staining) and CDK5 expression in salivary gland tumors samples of patients from four different subtypes. Black bar = both negative/ low expression of LMW-E and CDK5, blue bar = one positive/high LMW-E expression only, pink bar = one positive/high CDK5 expression only and yellow bar = both positive/high expression of both LMW-E and CDK5. p Values indicate a significant correlation between cyclin E (cytoplasmic staining) and CDK5 expression and were calculated by the Chi-square (\(\chi^2\)) test of association.