Fig. 5: LEF1 knockdown and over expression in K562 cells confirms influence of LEF1 on CCND1 expression.
From: PRMT6 activates cyclin D1 expression in conjunction with the transcription factor LEF1
A Western blot analysis of LEF1 knockdown in K562 cells. B Western blot analysis of LEF1 over expression in K562 cells. C mRNA level of LEF1 is decreased in LEF1 knockdown cells. D mRNA level of LEF1 is increased in LEF1 over expression cells. E CCND1 mRNA expression decreased after LEF1 knockdown. F mRNA level of CCND1 after LEF1 over expression in K562 cells. G BCL6 mRNA expression decreased after LEF1 knockdown. H mRNA level of BCL6 after LEF1 over expression in K562 cells. Error bars represent the standard deviation from the mean from at least three independent determinations. The P-values were calculated using Student’s t-test. *P < 0.05; **P < 0.01; ***P < 0.001.
