Fig. 2: Stemness profile of MamBo cell lines.

A Dark-field micrographs of mammosphere formation assay. White bar corresponds to 200 μm. Mean number of mammospheres ± SEM (n = 4): MamBo89HER2stable, 23 ± 1; MamBo43HER2labile, 17 ± 1; MamBo38HER2loss, 69 ± 9; MamBo38HER2loss vs MamBo89HER2stable and MamBo43HER2labile cell lines, p < 0.01 by Student’s t-test. B Expression of HER2 and stemness markers CD24, CD44, Sca1 and CD29, in cells cultured under 2D-adherent conditions, measured by cytofluorimetric analysis.