Fig. 2: GNMT regulation by AR.
From: PI3K-regulated Glycine N-methyltransferase is required for the development of prostate cancer
A–B Effect of AR activation with 5α-dihydrotestosterone (DHT) (A) and AR inhibition by MDV3100 (B) on GNMT mRNA levels in a time course in LNCaP cells. C–D Effect of surgical castration of 6-month old Ptenpc−/− mice on Gnmt (C) and Nkx3.1 (D) mRNA expression monitored by qPCR (n = 8–10) (Cnt Control non-castrated, Cast Castrated). E GNMT mRNA levels in prostate cancer cell lines with AR activity (represented with grey dots) and with low or no activity (black dots) (n = 3). F GNMT mRNA expression upon doxycycline-inducible expression (24 h) of YFP-PTEN in PTEN-deficient LNCaP prostate cancer cells in the presence or absence of the AR antagonist MDV3100 (n = 3). Values are represented as mean with SEM. Statistics: One sample T-test (A, B, F); Mann–Whitney U-test (C, D) and Student T-test with Welch correction (E). p, p value; *p < 0.05; **p < 0.01; ***p < 0.001.
