Fig. 1: Depletion of NEDD9 in human NSCLC cell lines impairs glycolysis.
From: NEDD9 sustains hexokinase expression to promote glycolysis
A Seahorse results in A549 and H460 cells treated with siRNA to NEDD9 (red) or scrambled siRNA (SCR, blue). Glycolysis is determined by measuring lactic acid acidification of the media. Arrows indicate addition of glucose (green), oligomycin (gray), and 2DG (black). B–D. Quantitation of results from (A) to demonstrate differences in glycolytic rate (B), glycolytic capacity (C), and glycolytic reserve (D). E–H Experiments parallel those shown in (A–D), but in conditions of 48 h of overexpressed NEDD9 (green) contrasted to cells transfected with empty vector (e.v., blue). I–L Experiments parallel those in (A–D), but in cells treated with vehicle or dasatinib. ECAR, extracellular acidification rate. Graphs shown represent average of three independent experiments. *p < 0.05, **p < 0.01; error bars represent SEM.
