Fig. 3: SIK1 is essential for DSRCT cell growth.
From: Salt-Inducible Kinase 1 is a potential therapeutic target in Desmoplastic Small Round Cell Tumor
A A673, BER-DSRCT, and JN-DSRCT-1 cells were transfected with two independent siWT1, siSIK1, or siScramble (control). Cell viability was measured 3 days after transfection. (*p < 0.05**p < 0.01, ***p < 0.001, ****p < 0.0001, mean ± SEM, student t-test). B Relative mRNA expression of SIK1 after 3 days of doxycycline treatment (****p < 0.0001, mean ± SEM, student t-test). C Western blot analyses showing SIK1 is effectively reduced in Dox-treated shSIK1 JN and BER-DSRCT cells compared to untreated or control cells. D Colony formation assay of dox-inducible shRenilla, shWT1, or shSIK1 DSRCT cell lines after 14 days of dox treatment (****p < 0.0001, mean ± SEM, student t-test). The right panel shows quantification of the stained cells in the colony formation assay.
