Fig. 1: Estradiol (E2) decreases p53 expression and is related to lung cancer progression.
Cancer development was induced by doxycycline treatment for 1.5 months in mice with EGFRL858R-induced lung cancer mice. The tumor areas in 12 mice including 4 control (RO, 2 male + 2 female), 4 male, and 4 female doxycycline-treated mice, were quantitated (A). The ovaries were removed from three female mice with EGFRL858R-induced lung cancer mice by ovariectomy, and mice were then sacrificed for H&E staining (B). A549 and PC14 cells with or without E2 treatment were used to study the level of p53 by IB with anti-p53 antibodies. After three independent experiments were completed, the level of p53 was quantified, and statistical assays were performed by a t tests; *P < 0.01, ***P < 0.001 (C). RNA samples were extracted from A549 cells with or without E2 treatment to study the global gene expression by RNA-sequencing assay. Up- and downregulated genes are listed (D, a); among these E2-regulated genes, p53-regulated genes are listed (D, b).
