Fig. 5: NNMT regulates EGFR expression and the Warburg effect in iCCA cells.

A Heat map of differentially expressed genes in HCCC-9810-Control and HCCC-9810-NNMT cells. B The expression level of the EGFR mRNA in iCCA and adjacent tissues. C The mRNA expression level was used to determine the correlation between NNMT and EGFR expression. D The expression level of the EGFR protein in iCCA and adjacent tissues. E WB was used to detect the expression levels of the EGFR and p-EGFR protein in iCCA cells transfected with NNMT lentivirus. F Colony formation assays detected the proliferation of iCCA cells transfected with si-EGFR. G Transwell assays detected the migration of iCCA cells transfected with si-EGFR. H Transwell assays detected the invasion of iCCA cells transfected with si-EGFR. Scale bars: 100 μm. I In vivo experiments were conducted to detect the effect of si-EGFR on tumor proliferation in nude mice. J The Seahorse X96 analyzer detects the acid excretion capacity and oxygen consumption capacity of iCCA cell lines. K–M lactic acid and ATP production and glucose consumption in iCCA cells transfected with si-EGFR. N The Kaplan–Meier method was used to determine the overall survival rate of patients with iCCA (data are mean ± SEM, *P < 0.05, **P < 0.01, ***P < 0.001, n = 3).