Fig. 6: NNMT induces EGFR expression by decreasing the levels of H3K9me3 and H3K27me3. | Oncogenesis

Fig. 6: NNMT induces EGFR expression by decreasing the levels of H3K9me3 and H3K27me3.

From: NNMT promotes the progression of intrahepatic cholangiocarcinoma by regulating aerobic glycolysis via the EGFR-STAT3 axis

Fig. 6: NNMT induces EGFR expression by decreasing the levels of H3K9me3 and H3K27me3.

A Co-IP analysis of the binding between NNMT and EGFR in HCCC-9810 cells transfected with the Flag-NNMT lentivirus (left panel). Co-IP analysis of the binding between NNMT and EGFR in HCCC-9810 cells transfected with the EGFR plasmid (right panel). B, C HPLC detected the levels of SAM and SAH and the ratio of SAM/SAH in iCCA cells. D WB analysis of the effect of NNMT on the levels of H3K9me3 and H3K27me3 in iCCA cells. E, F The effect of SAM (500 μM) or methionine (100 μM) on the methylation level and EGFR expression in iCCA cells. G WB detection and analysis of the EGFR protein level in cells treated with 5 μM UNC0646 or 5 μM GSK126 in the culture medium. H, I ChIP analyses of the degree of enrichment of H3K9me3 (left panel) or H3K27me3 (right panel) at the EGFR promoter region (data are mean ± SEM, *P < 0.05, **P < 0.01, ***P < 0.001, n = 3).

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