Fig. 6: Loss of ZNF148 increases breast cancer cell stemness.
From: A MYC-ZNF148-ID1/3 regulatory axis modulating cancer stem cell traits in aggressive breast cancer
A Representative flow cytometry plots and a bar graph, showing the percentage of viable Aldehyde dehydrogenase bright (ALDHbr) shCtr and shZNF148 MDA-MB-231 cells in the presence (+) or absence (−) of diethylaminobenzaldehyde (DEAB) (n = 3). B As in “A” showing the percentage of ALDHbr cells in MDA-MB-231 Control and MDA-MB-231-ZNF148KO cells (n = 5). C Representative image of clonogenic assay of cells in “B” stained with crystal violet (n = 3). Scale bar = 1 cm. Error bars represent mean ± SD. Student’s t-test, *P < 0.05. SSC side scatter; ALDHbr Aldehyde dehydrogenase bright; Ctr Control; KO knockout; DEAB diethylaminobenzaldehyde.
