Fig. 2: Vortioxetine hydrobromide bound with JAK2 and SRC.

A The interaction between vortioxetine hydrobromide (pink) and JAK2 (green) was predicted using a computational docking model. Hydrogen bonds were represented as a yellow dash line. B The interaction between vortioxetine hydrobromide (pink) and SRC (blue) was predicted using a computational docking model. Hydrogen bonds were represented as a yellow dash line. C–E Vortioxetine hydrobromide directly bound to JAK2 and SRC. The recombinant proteins (C) or GC cell lysates (D, E) were incubated with vortioxetine hydrobromide-conjugated Sepharose 4B beads or with Sepharose 4B beads alone. The results were analyzed by Western blotting. Data from three independent experiments were shown. The binding capacity of vortioxetine hydrobromide to JAK2 and SRC in AGS (F) and HGC27 (G) intact cells. The cells were treated with vortioxetine hydrobromide or DMSO for 24 h and incubated in different temperatures. The protein bindings were visualized by Western blotting. Mean ± S.D. (n = 3).