Fig. 2: Anastasis promotes tumor growth and metastasis.

A Image of xenografts formed by BT474-ADR-ZsGreen⁺, BT474-ADR-ZsGreen⁻ and BT474-control cells in nude mice. B On the left are the growth curves of the xenografts. * Indicates significant difference compared to the control group. # Indicates significant difference compared to the ADR-ZsGreen⁻ group. The bar graph on the right shows the weights of the xenografts in (A). n = 8. C Ki-67 expression in xenografts formed by the indicated cell populations derived from BT474 cells. Left: representative images of immunohistochemistry (IHC) staining. Scale bar, 50 μm. Right: quantification of the percentage of Ki-67⁺ cells in tumors. n = 5. D Image of xenografts formed by MDA-MB-231-ADR-ZsGreen⁺, MDA-MB-231-ADR-ZsGreen⁻ and MDA-MB231-control cells in nude mice. E On the left are the growth curves of the xenografts. * Indicates significant difference compared to the control group. # Indicates significant difference compared to the ADR-ZsGreen⁻ group. The bar graph on the right shows the weights of the xenografts in (D). n = 8. F Ki-67 expression in xenografts formed by the indicated cell populations derived from MDA-MB-231 cells. Left: representative images of IHC staining. Scale bar, 50 μm. Right: quantification of the percentage of Ki-67⁺ cells in tumors. n = 5. G Lung metastasis of MDA-MB-231-ADR-ZsGreen⁺, MDA-MB-231-ADR-ZsGreen⁻ and MDA-MB-231-control cells. Left: the representative images of the lungs and H & E staining. Black arrows in the upper row point to examples of tumor nodules. Scale bars in the lower row are 100 µm. Right: the number of metastatic tumor nodules in lungs from each mouse. n = 8. Data are presented as mean ± SEM. Statistical significance was determined using one-way ANOVA with Tukey test. * or # P < 0.05, ** or ## P < 0.01, *** or ### P < 0.001.